The best replacement for trypan blue is methylene blue.

The best alternatives to trypan blue are rated as following:
★★★★★  methylene blue
★★★★  propidium iodide
★★★  mtt
★  brilliant blue
★  evans blue

Your alternative for trypan blue is not listed? Suggest!
Speaking about trypan blue, one probably shouldn't forget about adherent cells.

Other search terms related to trypan blue and their ratings are:
★★★★★ adherent cells
You have in mind a companion for trypan blue and it is not yet here? Propose!



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trypan blue
methylene blue
propidium iodide