The best replacement for rt-pcr is pcr.


The best alternatives to rt-pcr are rated as following:
★★★★★  pcr
★★★★  qpcr
★★★★  northern blot
★★★  rna-seq
★★  qrt-pcr
★★  rt-qpcr
★  microarray
  conventional pcr
  real time pcr

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No companions for rt-pcr have been suggested so far.
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Google Trends

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rt-pcr
pcr
qpcr