The best replacement for elisa is western blot.

The best alternatives to elisa are rated as following:
★★★★★  western blot
★★★★  eia
★★★  pcr
★★  ria
★★  ifa
★★  elispot
★  alcat
★  immunoblot
★  western blot test
★  western blot hiv
  flow cytometry
  rapid test
  rast
  rapid hiv test
  hplc
  sandwich elisa
  luminex
  radioimmunoassay
  eia assay
  indirect elisa
  western blotting
  ihc
  western blot sensitivity
  immunofluorescence
  immunoblotting
  xbmc
  agid coggins
  eia hiv
  elfa
  immunoassay
  immunohistochemistry
  emit
  western blot tests
  chemiluminescence

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Speaking about elisa, one probably shouldn't forget about western blot test.

Other search terms related to elisa and their ratings are:
★★★★★ western blot test
★★★★ western blot
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